This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.

Search for Publication

Year(s) from:  to 
Keywords (separated by spaces):

Comparison of microfocus- and synchrotron X-ray tomography for the analysis of osteointegration around Ti6Al4V-implants

R. Bernhardt, D. Scharnweber, B. Müller, P. Thurner, H. Schliephake, P. Wyss, F. Beckmann, J. Goebbels and H. Worch
European Cells and Materials
Vol. 7, pp. 42-51, 2004


Micro-computed tomography (µCT) provides quantitative three-dimensional information of bone around titanium implants similar to classical histology. The study, based on an animal model, containing cuboid-shaped biofunctionalised Ti6Al4V implants with surrounding bone after 4 weeks, is performed using 3 µCT-systems with X-ray tubes, one synchrotron-radiation-based µCT-system (SRµCT), and classical histology. Although the spatial resolution of the µCT-systems is comparable, only the results of SRµCT agree with results of classical histology. The X-ray tube sources give rise to huge artefacts in the tomograms (interface scattering, beam hardening), which impaired the quantitative analysis of bone up to about 200 µm from the implant surface. Due to the non-destructive character of µCT the specimens can be subsequently examined by classical histology without restriction. The quantitative comparison of bone formation uncovers the strong dependence of the newly formed bone from the selected slice. This implies the necessity of 3D analysis. SRµCT and classical histology prove that surface modifications of the titanium implant significantly influence the bone formation. Using SRµCT, the preparation artefacts due to cutting and polishing are excluded.

Download in pdf format
  author = {R. Bernhardt and D. Scharnweber and B. M\"uller and P. Thurner and H. Schliephake and P. Wyss and F. Beckmann and J. Goebbels and H. Worch},
  title = {Comparison of microfocus- and synchrotron X-ray tomography for the analysis of osteointegration around Ti6Al4V-implants},
  journal = {European Cells and Materials},
  year = {2004},
  month = {},
  pages = {42-51},
  volume = {7},
  number = {},
  keywords = {X-ray micro computed tomography, comparison of microfocus and synchrotron radiation, surface modification of titanium implants, histology, bone segmentation, quantification of bone formation}